TE7细胞

是否是肿瘤细胞： 0

物种来源： 小鼠

细胞形态： **样

**类型： IgG1

运输方式： 冻存运输

生长状态： 悬浮生长

ATCC Number： HB-208™

数量： 大量

Designations: TE7

Depositors: BF Haynes

Isotype: IgG1

Biosafety Level: 1

TE7细胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human thymus

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Comments: Animals were immunized with human thymic stroma.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with cells in the thymic capsule and interlobular septae and vessels.

It also reacts with non-endocrine fibrous stroma in the thymus, and with mesodermally derived connective tissue in other parts of the body.

It defines the mesodermal component of the human thymic microenvironment that most likely induces thymic epithelial differentiation early in the development of the thymus.

TE7细胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1638: Haynes BF, et al. TE7细胞Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562