年限： *****
细胞形态： 上皮样
ATCC Number： CCL-42™
生长状态： 贴壁生长
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 其他
组织来源： connective tissue and muscle
Designations: FHM
FHM细胞Depositors: RG Malsberger
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Pimephales promelas
Morphology: epithelial
Source: Tissue: connective tissue and muscle
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Isolation: Isolation date: April, 1962
Virus Resistance: poliovirus 1
Age: *****
Comments: The FHMFHM细胞 (Fat Head Minnow) line of epithelial cells was derived from tissue posterior to the anus, exclusive of the caudal fin, of normal ***** minnows of both sexes by M. Gravell and R.G. Malsberger, in April, 1962. [22174]
The cells multiply over a wide range of temperatures (0 to 36C) with optimal growth between 28C and 34C.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Hanks' BSS, 90%; fetal bovine serum, 10%
Atmosphere: air, 100%
Temperature: 34.0°C; (Max. 34.0 °C, Min. 28.0°C)
Subculturing: Protocol: Remove old medium, add fresh 0.25% trypsin solution, rinse and remove. Add 1 to 2ml of trypsin solution, allow the flask to sit at room temperature until the cells detach. FHM细胞Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: At the time of subcultivation (this will vary depending upon the temperature of incubation)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 22174: Gravell M, Malsberger RG. A permanent cell line from the fathead minnow (Pimephales promelas). Ann. N.Y. Acad. Sci. 126: 555-565, 1965. PubMed: 5220177
26198: Leibovitz A. FHM细胞The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am. J. Hyg. 78: 173-180, 1963. PubMed: 14063721
