MD2细胞

ATCC Number： HB-229™

细胞形态： **样

**类型： IgM

生长状态： 悬浮生长

运输方式： 冻存运输

数量： 大量

是否是肿瘤细胞： 0

物种来源： 大鼠

Designations: MD2

Depositors: RV Rouse

Isotype: IgM

MD2细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus MD2细胞(myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse medullary thymic epithelium

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with mouse thymic stromal tissue.

Spleen cells were fused with Sp2/0 myeloma cells.

MD2细胞In the mouse thymus, MD2 is specific for dendritic cells in the medulla.

The antibody cross-reacts with rare cells in the human thymic medulla and faintly with Hassall's bodies.

It also reacts with epithelial elements in a variety of mouse tissues.

Propagation: ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 58334: Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. MD2细胞PubMed: 2461413