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Kasumi-1细胞

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产品名称: Kasumi-1细胞
产品型号: Kasumi-1
产品展商: HZbscience
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简单介绍

Kasumi-1细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。Kasumi-1细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


Kasumi-1细胞  的详细介绍

Kasumi-1细胞

ATCC Number: CRL-2724™

相关**: 其他**

器官来源: 外周血

运输方式: 冻存运输

细胞形态: 原始粒细胞

生长状态: 悬浮生长

年限: 7 yrs juvenile

数量: 大量

细胞类型: 其他细胞类型

是否是肿瘤细胞: 0

物种来源: 人

Designations: Kasumi-1

Depositors: H Asou

Biosafety Level: 1

Shipped: frozen

Kasumi-1细胞Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: myeloblast


Source: Organ: peripheral blood

Disease: acute myeloblastic leukemia

Cell Type: myeloblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: The cell line was established from the peripheral blood of an acute myeloid leukemia (AML) patient.

Kasumi-1细胞The cells are positive for myeloperoxidase showing a morphology of myeloid maturation.

This translocation juxtaposes the AML1 with ETO (or MTG8) gene, giving rise to the fusion gene AML1-ETO (also known as AML1-MTG or RUNX1-CBF2T1), hence the cells produce chimeric AML1-ETO protein.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 11,13

D16S539: 9,12

D5S818: 9,11

D7S820: 8,11

THO1: 6,9

TPOX: 8,9

vWA: 14

Cytogenetic Analysis: t(8:21) (q22;q22)

Age: 7 yrs juvenile

Gender: male

Ethnicity: Japanese

Comments: This is a leukemic cell line with an 8;21 chromosome translocation. Kasumi-1细胞This translocation juxtaposes the AML1 with ETO (or MTG8) gene, giving rise to the fusion gene AML1-ETO (also known as AML1-MTG or RUNX1-CBF2T1), hence the cells produce chimeric AML1-ETO protein. This protein down-regulates CEBPA mRNA, protein and DNA binding activity, which is crucial for the differentiation of granulocytes. The cell line was established from the peripheral blood of an acute myeloid leukemia (AML) patient. The cells are positive for myeloperoxidase showing a morphology of myeloid maturation. In proliferation assay the cells in culture showed response to interleukin-3 (IL-3), IL-6, granulocyte colony-stimulating factor (G-CSF), and granulocytemacrophage CSF (GM-CSF), but not to IL-1 or IL-5. Neither granulocytic nor eosinophilic maturation was observed in the in vitro liquid culture by the addition of dimethyl sulfoxide, G-CSF, or IL-5, respectively. Induction of macrophagelike cells was seen by the addition of phorbol ester. Proliferation is inhibited by 1,25S-(OH)2-16,23-diene-26-F3-10-nor D3.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(5) viable cells/ml.

Interval: Maintain cell density between 3 X 10(5) and 3 X 10(6) viable cells/ml.

Medium Renewal:Kasumi-1细胞 Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 40 to 48 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

Bioreactive Factors: Differentiation Inducers: 12-O-tetradecanoylphorbol-13-acetate (TPA)

References: 57589: Tashiro S, et al. Establishment of a human acute myeloid leukemia cell line (Kasumi-1) with 8;21 chromosome translocation. Blood 77: 2031-2036, 1991. PubMed: 2018839

61289: Miyoshi H, et al. The t(8;21) translocation in acute myeloid leukemia results in production of an AML1-MTG8 fusion transcript. EMBO J. 12: 2715-2721, 1993. PubMed: 8334990

61291: Miyoshi H, et al. t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1. Proc. Natl. Acad. Sci. USA 88: 10431-10434, 1991. PubMed: 1720541

61292: Asou H, et al. 19-nor vitamin-D analogs: a new class of potent inhibitors of proliferation and inducers of differentiation of human myeloid leukemia cell lines. Blood 92: 2441-2449, 1998. PubMed: 9746784

Kasumi-1细胞61293: Kozu T, et al. Junctions of the AML1/MTG8(ETO) fusion are constant in t(8;21) acute myeloid leukemia detected by reverse transcription polymerase chain reaction. Blood 82: 1270-1276, 1993. PubMed: 8353289

61295: Pabst T, et al. AML1-ETO downregulates the granulocytic differentiation factor C/EBPalpha in t(8;21) myeloid leukemia. Nat. Med. 7: 444-451, 2001. PubMed: 11283671

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