R1.G1细胞

R1.G1细胞

细胞类型： T**细胞

是否是肿瘤细胞： 0

物种来源： 小鼠

品系： C58/J

数量： 大量

运输方式： 冻存运输

器官来源： 胸腺

ATCC Number： TIB-44™

相关**： **瘤

细胞形态： **样

生长状态： 悬浮生长

Designations: R1.G1

Depositors: R Hyman

R1.G1细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: thymus

Strain: C58/J

Disease: lymphoma

Cell Type: T lymphocyte;

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Antigen Expression: H-2k; Thy-1.2; TL

Comments: This line was derived from R1.1 (ATCC TIB-42).

The cells are resistant to 0.1 mM 6-thioguanine.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22247: R1.G1细胞Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.