Fcwf-4细胞
年限： fetus
器官来源： 胚胎
细胞类型： 其他细胞类型
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 猫
生长状态： 贴壁生长
ATCC Number： CRL-2787™
细胞形态： 其他
Designations: Fcwf-4 [Fcwf]
Depositors: NC Pedersen
Fcwf-4细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Felis catus
Morphology: spindle to stellate
Source: Organ: fetus, whole
Cell Type: macrophage
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Isolation: Isolation date: 1979
Virus Susceptibility: Feline coronavirus
Age: fetus
Comments: This continuous feline cell line was developed in 1979. It is used to propagate tissue culture adapted Feline coronavirus (Feline infectious peritonitis virus, FIPV). The cells possess some characteristics of macrophages (nonspecific esterase, phagocytic activity, Fc receptors).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Fcwf-4细胞Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
An inoculum of 1 X 10(4) to 1 X 10(5) viable cells/cm2 is recommended. Do not exceed 1 X 10(6) cells/cm2..
Incubate cultures at 37�C.
Interval: Maintain cultures at a cell concentration between 2 X 10(4) and 1 X 10(5) cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: Fcwf-4细胞Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 31 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 71888: Jacobse-Geels HE, Horzinek MC. Expression of feline infectious peritonitis coronavirus antigens on the surface of feline macrophage-like cells. J. Gen. Virol. 64: 1859-2866, 1983. PubMed: 6886678
Fcwf-4细胞71889: Pedersen NC, et al. Infection studies in kittens, using feline infectious peritonitis virus propagated in cell culture. Am. J. Vet. Res. 42: 363-367, 1981. PubMed: 6267959
