TE3 [TE3A]细胞

TE3 [TE3A]细胞

细胞形态： **样

**类型： IgG2

是否是肿瘤细胞： 0

物种来源： 小鼠

运输方式： 冻存运输

生长状态： 悬浮生长

ATCC Number： HB-209™

TE3 [TE3A]细胞数量： 大量

Designations: TE3 [TE3A]

Depositors: BF Haynes

Isotype: IgG2

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human thymus (cortical thymic epithelium)

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Comments: Animals were immunized with human thymic stroma.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with an intracellular antigen expressed in cortical, but not medullary, thymic epithelium.

All TE3+ cells in the thymus were found to express HLA and Ia antigens, and contain keratin.

Propagation: ATCC complete growth medium: TE3 [TE3A]细胞Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1639: McFarland EJ, et al. The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. TE3 [TE3A]细胞J. Immunol. 133: 1241-1249, 1984. PubMed: 6205074