4A6细胞
细胞形态： **样
**类型： IgM
运输方式： 冻存运输
生长状态： 悬浮生长
ATCC Number： CRL-1928™
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: 4A6
Depositors: JE Rothman
Isotype: IgM
Biosafety Level: 1
4A6细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against golgi vesicular transport protein
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Comments: 4A6细胞Animals were immunized with purified N-ethylmaleimide (NEM) sensitive factor.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with an NEM sensitive fusion protein required for vesicular transport through the golgi stack.
The antibody should not be frozen, and should be used in buffers containing at least 65 mM KCl to avoid precipitation.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, 4A6细胞add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1779: Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011
23306: Block MR, et al. Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport. Proc. Natl. Acad. Sci. USA 85: 7852-7856, 1988. 4A6细胞PubMed: 3186695
