PC-12细胞
生长状态： 漂浮生长
器官来源： 肾上腺
是否是肿瘤细胞： 0
物种来源： 大鼠
运输方式： 冻存运输
数量： 大量
细胞形态： 其他
ATCC Number： CRL-1721™
相关**： 其他**
Designations: PC-12
Depositors: B Patterson
PC-12细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: floating clusters; few scattered lightly attached cells.
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: small irregularly shaped cells
Source: Organ: adrenal gland
Disease: pheochromocytoma
Cellular Products: catecholamines; dopamine; norepinephrine
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Applications: transfection host
Receptors: nerve growth factor (NGF), expressed
Tumorigenic: Yes
Cytogenetic Analysis: 40 chromosomes; 38 autosomes plus XY [1163]
Gender: PC-12细胞male
Comments: The PC-12 cell line was derived from a transplantable rat pheochromocytoma.
The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks.
The cells do not synthesize epinephrine.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
heat-inactivated horse serum to a final concentration of 10%
fetal bovine serum to a final concentration of 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used for this protocol are for a 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Transfer cell suspension to centrifuge tube. Centrifuge cells at 180 to 225 xg for 8-15 minutes at room temperature.
Remove and discard supernatant leaving cell pellet.
Resuspend the cell pellet in an appropriate volume of fresh medium (about one tenth of the original volume.
PC-12细胞Gently aspirate each 5 ml aliquot of cells 4 or 5 times with a new 20 ml syringe outfitted with a 22g (1� in.) needle to break up cell clusters.
Add appropriate aliquots of the cell suspension to new 75 cm2 flask with 10-15 ml fresh growth medium. Seed flask 5 x 10(5) to 1 x 10(6) viable cells/ml or use subcultivation ratio of 1:2 to 1:4.
Place culture vessels in incubator at 37�C Subculture when cell density reaches between 2-4 x 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 48 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
Related cell line: ATCC CRL-1721.1, PC-1
References: 1162: Levi A, et al. PC-12细胞Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317
1163: Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
22344: Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712
33014: Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125
16173681: U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005
