LADMAC细胞
器官来源： 骨髓
细胞形态： **样
ATCC Number： CRL-2420™
数量： 大量
年限： *****
生长状态： 悬浮生长，少量贴壁
品系： C3H
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
Designations: LADMAC
LADMAC细胞Depositors: WS Walker
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, with some loosely adherent cells
Organism: Mus musculus deposited as mouse
Morphology: lymphoblast
Source: Organ: bone marrow
Strain: C3H
Cellular Products: colony stimulating factor-1 (CSF-1)
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Tumorigenic: Yes
Age: *****
Comments:LADMAC细胞 LADMAC is a transformed cell line derived by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with cloned human cellular myc-homologous sequences covalently attached to pBR325 (pR myc).
The cell line has monocyte-like morphology; contains nonspecific esterase; is phagocytic for latex beads; secretes lysozyme, and bears the Mac-1 antigen.
A minority of cells are Fc receptor positive and an appreciable number of cells are complement receptor 1 positive.
The cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. The cells are not phagocytic for antibody or complement-coated particles; they do not constitutively secrete Interleukin-1.
LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. [38883]
The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1. [38884]
This cell line is used to produce LADMAC conditioned medium. It will support the growth of the macrophage cell lines EOC 2 (CRL-2467), EOC 13.31 (CRL-2468), EOC 20 (CRL-2469), I-11.15 (CRL-2470) and I-13.35 (CRL-2471).
Propagation: ATCC complete growth medium: LADMAC细胞The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 38883: Sklar MD, et al. LADMAC细胞Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730
38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
