MC2/3细胞
ATCC Number： CRL-2143™
运输方式： 冻存运输
生长状态： 贴壁生长
细胞形态： 成纤维样
数量： 大量
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 仓鼠
Designations: MC2/3
Depositors: RL Stallings
Biosafety Level: 1
MC2/3细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cricetulus griseus
Morphology: fibroblast
Source: Cell Type: somatic cell hybrid;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.MC2/3细胞 Please click here for information regarding the specific requirements for shipment to your location.
Comments: MC2/3 is a mouse chromosome 8 specific monochromosome hybrid cell line.
This hybrid resulted from the fusion of adenine phosphoribosyl transferase negative (APRT -) Chinese hamster ovary cells with micronuclei from near euploid thymidine kinase negative (TK -) CAK mouse cells.
Hybrids containing mouse chromosome 8 (which has the APRT locus) were selected in medium containing 0.075 mM adenine, 800 nM aminopterin and 0.016 mM thymidine (AAT).
MC2/3细胞The mouse chromosome 8 could be from either the C57BL/6J or ARK/J inbred strain, since CAK cells were derived from an F1 hybrid of those two strains.
Mouse chromosome 8 may be isolated with 96% purity.
The depositor has reported the presence of one copy of mouse chromosome 8 in each Chinese hamster cell by G band cytogenetics, fluorescence in situ hybridization (FISH) and PCR analysis.
However, FISH analysis of ATCC frozen stocks by the depositor using biotinylated total genomic mouse DNA as probe indicates that 7 of 9 metaphases examined had a single mouse acrocentric chromosome present in a hamster background.
Two of nine metaphases showed different rearrangements of the mouse acrocentric.
An examination of interphase nuclei indicates that about 18% of the cells are tetraploid showing two distinct hybridization signals from the mouse while 82% are near diploid exhibiting one distinct signal.
The level of tetraploidy may increase with additional passage.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium containing 0.075 mM adenine, 800 nM aminopterin, and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: MC2/3细胞Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22418: Stallings RL, Deaven LL. A mouse monochromosome 8 somatic cell hybrid: a reagent for chromosome 8 isolation. Mamm. Genome 5: 572-573, 1994. PubMed: 8000142
