YB2/0 [YB2/3HL.P2.G11.16Ag.20]细胞
细胞形态： **样
运输方式： 冻存运输
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 大鼠
ATCC Number： CRL-1662™
数量： 大量
Designations: YB2/0 [YB2/3HL.P2.G11.16Ag.20]
Depositors: C Milstein
YB2/0 [YB2/3HL.P2.G11.16Ag.20]细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphoblast;
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Comments: YB2/0 [YB2/3HL.P2.G11.16Ag.20]细胞This line was produced by fusing Y3/Ag1.2.3 (ATCC CRL-1631) cells with spleen cells from an AO strain rat.
The cells do not secrete either heavy or light immunoglobulin chains.
The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: YB2/0 [YB2/3HL.P2.G11.16Ag.20]细胞Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
References: 1094: Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596
22292: . . YB2/0 [YB2/3HL.P2.G11.16Ag.20]细胞Methods Enzymol. 73B: 1-46, 1981.
