4F2C13细胞
运输方式： 冻存运输
细胞形态： **样
**类型： IgG2a; kappa light chain
数量： 大量
ATCC Number： HB-22™
是否是肿瘤细胞： 0
物种来源： 小鼠
4F2C13细胞生长状态： 悬浮生长
Designations: 4F2C13
Depositors: A Fauci, BF Haynes
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: 4F2C13细胞lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human monocyte
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with HSB-2 cells (ATCC CCL-120.1, an acute lymphocytic T leukemia cell line).
Spleen cells were fused with P3X63Ag8 myeloma cells.
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21.
4F2C13细胞The antibody reacts with peripheral blood monocytes and to a lesser extent peripheral blood T cells.
The antibody fixes complement and binds to Protein A.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; 4F2C13细胞carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 916: Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. 4F2C13细胞PubMed: 6153676
