BLK CL.4细胞

BLK CL.4细胞

细胞形态： 成纤维样

生长状态： 贴壁生长

ATCC Number： TIB-81™

数量： 大量

相关**： 正常

品系： C57BL/6

细胞类型： 成纤维细胞

是否是肿瘤细胞： 0

物种来源： 小鼠

运输方式： 冻存运输

BLK CL.4细胞年限： embryo

Designations: BLK CL.4

Depositors: P Patek, J Collins, M Cohn

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Disease: normal

Strain: C57BL/6

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.BLK CL.4细胞 Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Age: embryo

Comments: These cells do not grow in 0.3% agarose.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. BLK CL.4细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove old medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

References: 22905: Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. BLK CL.4细胞PubMed: 723934