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HEL 92.1.7细胞

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产品名称: HEL 92.1.7细胞
产品型号: HEL 92.1.7
产品展商: HZbscience
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简单介绍

HEL 92.1.7细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。HEL 92.1.7细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


HEL 92.1.7细胞  的详细介绍

HEL 92.1.7细胞

ATCC Number: TIB-180™

相关**: 白血病

细胞类型: 其他细胞类型

运输方式: 冻存运输

数量: 大量

是否是肿瘤细胞: 0

物种来源: 人

生长状态: 悬浮生长

年限: 30 years

细胞形态: **样

HEL 92.1.7细胞器官来源: 骨髓

Designations: HEL 92.1.7

Depositors: PJ Martin

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast


Source: Organ: bone marrow

Disease: erythroleukemia

Cell Type: HEL 92.1.7细胞erythroblast

Cellular Products: hemoglobin; globin (G gamma, A gamma, epsilon, eta and alpha chains); beta-2-microglobulin; glycophorin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: These cells differentiate spontaneously into erythroblast-like cells.

Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid).

Antigen Expression: HLA A3, Aw32, Bw35; Ia+

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 9,11

D16S539: 11

D5S818: 11

D7S820: 7

THO1: 7

TPOX: 11

vWA: 14,17

Age: 30 years

HEL 92.1.7细胞Gender: male

Ethnicity: Caucasian

Comments: These cells differentiate spontaneously into erythroblast-like cells.

Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.

Related Products: HEL 92.1.7细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22614: Papayannopoulou T, et al. Human erythroleukemia cell line (HEL) undergoes a drastic macrophage- like shift with TPA. Blood 62: 832-845, 1983. PubMed: 6309287

23500: Martin P, Papayannopoulou T. HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science 216: 1233-1235, 1982. PubMed: 6177045

32395: Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679

32446: Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663

33012: Riewald M, et al. HEL 92.1.7细胞Identification and characterization of the cytoplasmic antiproteinase (CAP) in human platelets. J. Biol. Chem. 271: 7160-7167, 1996. PubMed: 8636153

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