BCL1 clone 5B1b细胞
ATCC Number： TIB-197™
相关**： 其他**
数量： 大量
生长状态： 贴壁生长
细胞类型： B**细胞
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
**类型： IgD; IgM; lambda light chain
BCL1 clone 5B1b细胞运输方式： 冻存运输
品系： BALB/c
Designations: BCL1 clone 5B1b
Depositors: S Strober
Isotype: IgD; IgM; lambda light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: BCL1 clone 5B1b细胞leukemia; lymphoma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin (surface, sIg +); monoclonal antibody
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Gender: female
Comments: The cells can be activated by LPS to produce IgM and can be used as a target for B cell growth factor (BCGF) assays.
Tested and found negative for ectromelia virus (mousepox).
Propagation: BCL1 clone 5B1b细胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine and 0.05 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Subcultures are prepared by scraping. Dislodge cells in the spent medium, aspirate, add fresh medium and dispense into new flasks.
References: 22831: Gronowicz ES, et al. An in vitro line of the B cell tumor BCL1 can be activated by LPS to secrete IgM1. J. Immunol. 125: 976-980, 1980. PubMed: 6157730
22835: Thoman ML, Weigle WO. Identification and partial purification of a B cell growth-promoting material utilizing the BCL1 cell line. J. Immunol. 130: 233-236, 1983. PubMed: 6600179
22845: Strober S, et al. BCL1 clone 5B1b细胞Immunobiology of a spontaneous murine B cell leukemia (BCL1). Immunol. Rev. 48: 169-195, 1979. PubMed: 121100
22904: Slavin S, Strober S. Spontaneous murine B-cell leukaemia. Nature 272: 624-626, 1978. PubMed: 306069
