Mab 108细胞
细胞形态： **样
**类型： IgG2a kappa , IgG2a; kappa light chain
ATCC Number： HB-9764™
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
Designations: Mab 108
Mab 108细胞Depositors: Rorer Biotechnology, Inc.
Isotype: IgG2a kappa , IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human epidermal growth factor (EGF) receptor
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Comments: Animals were immunized with CH 71 Chinese hamster ovary cells that had been transfected with a plasmid bearing a truncated form of the epidermal growth factor receptor (EGFR) cDNA. [53501]
Spleen cells were fused with NS-1 mouse myeloma cells.
The antibody is specific for the human epidermal growth factor receptor and is capable of inhibiting the growth of human tumor cells expressing human epidermal growth factor receptors. [53501]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Mab 108细胞Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 53501: Schlessinger J, et al. Mab 108细胞Monoclonal antibodies specific to human epidermal growth factor receptor and therapeutic methods employing same. US Patent 6,217,866 dated Apr 17 2001
