PS 60细胞
生长状态： 悬浮生长
数量： 大量
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
**类型： IgG2b; kappa light chain
运输方式： 冻存运输
ATCC Number： CRL-1800™
Designations: PS 60
Depositors: H Gainer
Isotype: IgG2b; kappa light chain
PS 60细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against rat oxytocin associated neurophysin (NP-OT)
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Comments:PS 60细胞 Animals were immunized with an acid soluble extract of rat (Sprague-Dawley) intermediate lobes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for NP-OT of rat and other species (murine, human, bovine) in both radioimmunoassays and immunohistochemical staining.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
PS 60细胞heat-inactivated fetal bovine serum to a final concentration of 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1620: Ben-Barak Y, et al. Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies. J. Neurosci. 5: 81-97, 1985. PubMed: 3880813
2123: Ben-Barak Y, et al. Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin. Cell. Mol. Neurobiol. 4: 339-349, 1984. PS 60细胞PubMed: 6085284
