C58(NT)D.1.G.OUAR .1细胞
生长状态： 悬浮生长
ATCC Number： TIB-236™
相关**： **瘤
数量： 大量
运输方式： 冻存运输
细胞形态： **样
细胞类型： T**细胞
是否是肿瘤细胞： 0
物种来源： 大鼠
品系： WFu
C58(NT)D.1.G.OUAR .1细胞Designations: C58(NT)D.1.G.OUAR.1
Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: WFu
Cell Type: T lymphocyte;
Permits/Forms: C58(NT)D.1.G.OUAR .1细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
The line may be useful for the production of rat - rat T cell hybridomas.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: C58(NT)D.1.G.OUAR .1细胞Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 22645: Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313
22815: Geering G, et al. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J. Exp. Med. 124: 753-772, 1966. PubMed: 4288480
23167: Curling E, et al. C58(NT)D.1.G.OUAR .1细胞Isolation of rat/rat T cell hybrids using W/Fu C58(NT)D as fusion partner. J. Immunol. Methods 108: 171-178, 1988. PubMed: 2965188
