CE9H9细胞
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG1; kappa light chain
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
ATCC Number： HB-127™
CE9H9细胞Designations: CE9H9
Isotype: IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against insulin
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Comments: Animals were immunized with zinc containing crystalline human insulin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to insulin from human, cow, pig and rabbit and to pro-insulin from cow and pig.
The antibody cross-reacts with insulin from sheep, guinea pig, chicken and fish.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: CE9H9细胞The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 981: CE9H9细胞Schroer JA, et al. Mapping epitopes on the insulin molecule using monoclonal antibodies. Eur. J. Immunol. 13: 693-700, 1983. PubMed: 6193965
