Cf2Th细胞
生长状态： 贴壁生长
运输方式： 冻存运输
ATCC Number： CRL-1430™
相关**： 正常
是否是肿瘤细胞： 0
物种来源： 狗
年限： newborn
数量： 大量
器官来源： 胸腺
Cf2Th细胞Designations: Cf2Th
Depositors: WA Nelson-Rees
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Canis familiaris
Morphology:
Source: Organ: thymus
Disease: normal
Permits/Forms: Cf2Th细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Age: newborn
Gender: female
Comments: The line has been used to propagate retroviruses from cat embryo and baboon lung and kidney.
The cells are also susceptible to xenotropic retroviruses from AKR and BALB/c mice.
Propagation: Cf2Th细胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with non-essential amino acids, 80%; fetal bovine serum, 20%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22649: Nelson-Rees WA, et al. Source, alterations, characteristics and use of a new dog cell line (Cf2Th). In Vitro 12: 665-669, 1976. PubMed: 190163
32500: Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326
32728: Russell DW, Miller AD. Foamy virus vectors. J. Cf2Th细胞Virol. 70: 217-222, 1996. PubMed: 8523528
