36F-18C [36F]细胞
生长状态： 悬浮生长
数量： 大量
ATCC Number： HB-285™
运输方式： 冻存运输
细胞形态： **样
**类型： IgG2a
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: 36F-18C [36F]
36F-18C [36F]细胞Depositors: CR Mackay
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against sheep (ovine) CD2
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 36F-18C [36F]细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with phytohemagglutinin-stimulated sheep T cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for sheep CD2 and precipitates a 50000 to 55000 dalton molecule from lysates of sheep lymphocytes.
36F-18C [36F]细胞It does not inhibit cytotoxic T lymphocyte mediated killing or mixed lymphocyte reactivity.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23177: Mackay CR, et al. 36F-18C [36F]细胞Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol. 18: 1681-1688, 1988. PubMed: 2462499
