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39-S细胞

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产品名称: 39-S细胞
产品型号: 39-S
产品展商: HZbscience
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简单介绍

39-S细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。39-S细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


39-S细胞  的详细介绍

39-S细胞

ATCC Number: HB-8180™

数量: 大量

细胞形态: **样

**类型: IgG2a

生长状态: 悬浮生长

运输方式: 冻存运输

是否是肿瘤细胞: 0

物种来源: 小鼠

Designations: 39-S

39-S细胞Depositors: The United States of America

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against herpes simplex virus type 1 (HSV-1) internal capsid protein 8 (ICP 8)

Permits/Forms: 39-S细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with HSV-1.

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

The antibody is type specific for HSV-1 but does not neutralize the virus.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 85%; fetal bovine serum, 15% -OR- RPMI 1640 medium, 85%; fetal bovine serum, 15%

Subculturing: 39-S细胞Medium Renewal: Every 3 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1178: Showalter SD, et al. Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4. Infect. Immun. 34: 684-692, 1981. PubMed: 6277788

3544: Zweig M, et al. Monoclonal antibodies to herpes simplex virus type 1 polypeptides. 39-S细胞US Patent 4,572,896 dated Feb 25 1986

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