SJD.1细胞
ATCC Number： CRL-2296™
细胞形态： 成纤维样
数量： 大量
生长状态： 贴壁生长
是否是肿瘤细胞： 0
物种来源： 斑马鱼
器官来源： 其他
品系： SJD
年限： *****
运输方式： 冻存运输
Designations: SJD.1
SJD.1细胞Depositors: BH Paw, LI Zon
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Danio rerio
Morphology: fibroblast
Source: Organ: caudal fin
Strain: SJD
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: *****
Comments: SJD.1细胞SJD.1 is a fibroblast cell line derived from amputated caudal fins of an ***** zebrafish, strain SJD.
This strain of zebrafish is used for genetic mapping.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Temperature: 28.0°C
SJD.1细胞Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with trypsin (0.25%)-EDTA (0.03%)-polyvinylpyrrolidone (0.5%) solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: SJD.1细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 43315: Paw BH, Zon LI. Primary fibroblast cell culture. Methods Cell Biol. 59: 39-43, 1999. PubMed: 9891354
