SHM-D33细胞
细胞类型： 其他细胞类型
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 其他
数量： 大量
细胞形态： **样
ATCC Number： CRL-1668™
组织来源： heteromyeloma
生长状态： 悬浮生长
SHM-D33细胞Designations: SHM-D33
Depositors: HS Kaplan
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens; Mus musculus
Morphology: lymphoblast
Source: Tissue: heteromyeloma
Cell Type: SHM-D33细胞B lymphoblast; somatic cell hybrid
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Comments: This line was produced by fusing the human myeloma cell line FU-266, clone E-1 (HAT sensitive, 8-azaguanine resistant and resistant to G-418 - an antibiotic similar to gentamicin) with the murine myeloma P3X63Ag8.653 (see ATCC CRL-1580).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Modified Dulbecco's medium supplemented with 0.2 mg/ml G418 (Geneticin) and 20% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: SHM-D33细胞The cells are grown in 200 to 400 mcg/ml of G-418 to maintain selection for the human chromosomes.
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 4 X 10(4) viable cells/ml.
Interval: Maintain cultures at a cell concentraton between 3 X 10(4) and 8 X 10(5) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 992: Teng NN, et al. Construction and testing of mouse-human heteromyelomas for human monoclonal antibody production. Proc. Natl. Acad. Sci. USA 80: 7308-7312, 1983. PubMed: 6316357
1075: Kearney JF, et al. SHM-D33细胞A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 123: 1548-1550, 1979. PubMed: 113458
1180: Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745
