3Pt12B8细胞

3Pt12B8细胞

生长状态： 悬浮生长

ATCC Number： HB-8136™

是否是肿瘤细胞： 0

物种来源： 小鼠

数量： 大量

细胞形态： **样

**类型： IgM

3Pt12B8细胞运输方式： 冻存运输

Designations: 3Pt12B8

Depositors: Sidney Farber Cancer Institute

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

3Pt12B8细胞Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against T12 (120000 dalton) antigen on human T cells (T lymphocyte); against CD6

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Tumorigenic: Yes

Comments: Animals were immunized with human T chronic lymphocytic leukemia cells.

Spleen cells were fused with NS-1 myeloma cells.

The antibody has been used to prevent graft versus host disease (GVHD).

Propagation: 3Pt12B8细胞ATCC complete growth medium: Modified Dulbecco's medium; horse serum, 10%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 3512: Reinherz EL, Schlossman SF. Monoclonal antibody. 3Pt12B8细胞US Patent 4,443,427 dated Apr 17 1984