3G9F3细胞
ATCC Number: CRL-1843™
生长状态: 悬浮生长
细胞形态: **样
**类型: IgG2b
数量: 大量
是否是肿瘤细胞: 0
物种来源: 小鼠
运输方式: 冻存运输
Designations: 3G9F3
3G9F3细胞Depositors: CA Whetstone
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against pseudorabies virus (PRV)
Permits/Forms: 3G9F3细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with pseudorabies virus (IA strain 62-26).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
3G9F3细胞The antibody is specific for the gIII protein of PRV.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. 3G9F3细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
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