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3G9F3细胞

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产品名称: 3G9F3细胞
产品型号: 3G9F3
产品展商: HZbscience
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简单介绍

3G9F3细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。3G9F3细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


3G9F3细胞  的详细介绍

3G9F3细胞

ATCC Number: CRL-1843™

生长状态: 悬浮生长

细胞形态: **样

**类型: IgG2b

数量: 大量

是否是肿瘤细胞: 0

物种来源: 小鼠

运输方式: 冻存运输

Designations: 3G9F3

3G9F3细胞Depositors: CA Whetstone

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against pseudorabies virus (PRV)

Permits/Forms: 3G9F3细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with pseudorabies virus (IA strain 62-26).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

3G9F3细胞The antibody is specific for the gIII protein of PRV.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. 3G9F3细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

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