RG9/6.13 HLK细胞
细胞形态： **样
**类型： mouse IgG2b
器官来源： 脾
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： TIB-167™
运输方式： 冻存运输
数量： 大量
Designations: RG9/6.13 HLK
RG9/6.13 HLK细胞Depositors: TA Springer
Isotype: mouse IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against rat IgG2a (Fab')
Permits/Forms: RG9/6.13 HLK细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [58004]
Comments: Animals were immunized with pooled rat IgG.
Spleen cells were fused with NS-1 myeloma cells.
This antibody shows weak cross-reactivity with rat IgG2c and possibly IgG1.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: RG9/6.13 HLK细胞Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Adherent cells can be dislodged by scraping and cultures established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Subcultivation Ratio: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
References: 58004: Springer TA, et al. Monoclonal antibodies specific for rat IgG1, IgG2a and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. RG9/6.13 HLK细胞Hybridoma 1: 257-273, 1982. PubMed: 6208128
