EIAV 12E8.1细胞

EIAV 12E8.1细胞

数量： 大量

细胞形态： **样

**类型： IgG1

ATCC Number： HB-8917™

是否是肿瘤细胞： 0

物种来源： 小鼠

生长状态： 悬浮生长

运输方式： 冻存运输

EIAV 12E8.1细胞Designations: EIAV 12E8.1

Depositors: Fermenta Animal Health Company

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: EIAV 12E8.1细胞immunoglobulin; monoclonal antibody; against equine infectious anemia virus (EIA, EIAV) core antigen (p26)

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Comments: Animals were immunized with p26 prepared from the Wyoming strain of EIA virus.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Propagation: EIAV 12E8.1细胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 85%; fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: DMEM, 70%; fetal bovine serum, 20%; DMSO, 10%

References: 3914: Matsushita T, et al. Method for the detection of equine infectious anemia and other retrovirus infections using a competitive enzyme-linked immunoabsorbent assay and reagents useful in the same. EIAV 12E8.1细胞US Patent 4,806,467 dated Feb 21 1989