240-13D10细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
ATCC Number： CRL-2672™
数量： 大量
细胞形态： **样
**类型： IgG1 and IgG2b
运输方式： 冻存运输
240-13D10细胞Designations: 240-13D10
Depositors: National Cancer Institute
Isotype: IgG1 and IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: 240-13D10细胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a c-yes synthetic oncogene peptide
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Comments: Animals were immunized with a synthetic polypeptide with the sequence LMKLCWKKDPDERPTC corresponding to peptides 502 to 517 of the c-yes oncogene (formerly 770 to 793 of the v-yes oncogene).
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA tests, the antibody reacts strongly with synthetic peptides representing the yes, abl, and ros oncogenes. Weaker activity against src and fgr was also detected.
240-13D10细胞In immunoblot assays, a protein of approximately 70 kd is detected in a wide variety of cell lines.
The IgG1 reactivity of the antibody with the v-yes peptide (778 to 793) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium 240-13D10细胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
