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E.H. IV (Elaine IV)细胞

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产品名称: E.H. IV (Elaine IV)细胞
产品型号: E.H. IV (Elaine IV)
产品展商: HZbscience
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简单介绍

E.H. IV (Elaine IV)细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。E.H. IV (Elaine IV)细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


E.H. IV (Elaine IV)细胞  的详细介绍

E.H. IV (Elaine IV)细胞

运输方式: 冻存运输

细胞类型: B**细胞

是否是肿瘤细胞: 0

物种来源: 人

生长状态: 悬浮生长

ATCC Number: CCL-104™

相关**: 其他**

细胞形态: **样

数量: 大量

E.H. IV (Elaine IV)细胞器官来源: 外周血

年限: 20 years

Designations: E.H. IV (Elaine IV)

Depositors: W Henle

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast


Source: Organ: peripheral blood

Disease: infectious mononucleosis

Cell Type: E.H. IV (Elaine IV)细胞B lymphoblast;

Cellular Products: Epstein-Barr virus (EBV)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: partially resistant to poliovirus and vesicular stomatitis viruses

Isoenzymes: G6PD, B

Age: 20 years

Gender: female

Ethnicity: Caucasian

Comments: The cells carry and produce Epstein-Barr virus (EBV).

EBNA positive.

Biological Safety CLASS II.

Propagation:E.H. IV (Elaine IV)细胞 ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days

Cultures can be maintained by addition of fresh medium or by replacement of medium by sedimentation. Establish new cultures by seeding in fresh medium at 5 X 10 exp5 viable cells/ml.

References: 21415: Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134

26274: Niederman JC, et al. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. E.H. IV (Elaine IV)细胞JAMA 203: 205-209, 1968. PubMed: 4864269

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