E. Derm (NBL-6)细胞
生长状态： 贴壁生长
年限： 4 years
器官来源： 皮肤
ATCC Number： CCL-57™
运输方式： 冻存运输
细胞形态： 成纤维样
数量： 大量
品系： quarterhorse
组织来源： dermis
E. Derm (NBL-6)细胞是否是肿瘤细胞： 0
物种来源： 其他
Designations: E. Derm (NBL-6)
Depositors: AJ Kniazeff, WA Nelson-Rees, NB Darby
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Equus caballus
Morphology: fibroblast
Source: Organ: skin
Strain: E. Derm (NBL-6)细胞quarterhorse
Tissue: dermis
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: September, 1965
Virus Resistance: adenovirus 5; coxsackievirus A9, B5; poliovirus 2
Cytogenetic Analysis: model number = 64; range = 52 to 145. The donor's sex (female) is confirmed chromosomally. Although the chromosome numbers vary widely, 55% of the cells have a chromosome number within + or - 1 of the diploid and modal number (64).
Age: 4 years
Gender: female
Comments: Cells appear to senesce after 40 passages.
Propagation: E. Derm (NBL-6)细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: E. Derm (NBL-6)细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
