RAW 309F.1.1细胞

RAW 309F.1.1细胞

生长状态： 悬浮生长

数量： 大量

细胞形态： **样

运输方式： 冻存运输

是否是肿瘤细胞： 0

物种来源： 小鼠

器官来源： 脾

ATCC Number： TIB-51™

相关**： **瘤

RAW 309F.1.1细胞Designations: RAW 309F.1.1

Depositors: WC Raschke

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: spleen

Disease: lymphoma

Cellular Products: Abelson murine leukemia virus (infectious)

Permits/Forms: RAW 309F.1.1细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: H-2d; H-2b

Comments: The cells are negative for surface immunoglobulin (sIg-), Thy-1.2 (Thy-1.2-) and Ia (Ia-).

The cells do not pinocytose neutral red, phagocytose zymosan or latex beads and do not secrete lysozyme.

The line was derived from the RAW 309 tumor (see also ATCC TIB-69).

RAW 309F.1.1细胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1207: Raschke WC, et al. RAW 309F.1.1细胞Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198