26-8-16S细胞

26-8-16S细胞

ATCC Number： HB-42™

生长状态： 悬浮生长

运输方式： 冻存运输

细胞形态： **样

**类型： IgM

是否是肿瘤细胞： 0

物种来源： 小鼠

数量： 大量

Designations: 26-8-16S

26-8-16S细胞Depositors: DH Sachs, K Ozato

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against I-A k; cytotoxic

Permits/Forms: 26-8-16S细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with spleen cells from A.TL mice.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 26-8-16S细胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: Recommended medium 26-8-16S细胞(without the additional supplements or serum described under ATCC Medium):ATCC 46-X