SA13细胞
数量： 大量
细胞形态： **样
**类型： IgG; kappa light chain
ATCC Number： HB-8501™
运输方式： 冻存运输
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 人
Designations: SA13
Depositors: Cetus Corp.
SA13细胞Isotype: IgG; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: human (B cell); human (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against tetanus toxoid
Permits/Forms: SA13细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 9,10,12
THO1: 8,10,9.3
TPOX: 8,10,11
vWA: 16,17,20
SA13细胞Comments: Peripheral blood B cells from a patient that had been immunized with tetanus toxoid were fused with LTR228 lymphoblastoid cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 3570: Raubitschek AR, et al.SA13细胞 Human lymphoblastoid cell line and hybridomas derived therefrom. US Patent 4,624,921 dated Nov 25 1986
