200-3-G6-4 (20.4)细胞
ATCC Number： HB-8737™
数量： 大量
运输方式： 冻存运输
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG1
200-3-G6-4 (20.4)细胞Designations: 200-3-G6-4 (20.4)
Depositors: Wistar Institute
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 200-3-G6-4 (20.4)细胞immunoglobulin; monoclonal antibody; against nerve growth factor (NGF) receptor of primates
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Tumorigenic: Yes
Comments: Animals were immunized with human melanoma cell lines.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody binds to the 75000 dalton NGF receptor of primates.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: every 2 to 3 days
Preservation: 200-3-G6-4 (20.4)细胞Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
References: 3939: Koprowski H, et al. Diagnostic method for detection of neural crest disease. US Patent 4,786,593 dated Nov 22 1988
3940: Johnson EM Jr.. Tumor diagnostic method. US Patent 4,855,241 dated Aug 8 1989
23378: Levi AD, et al. The functional characteristics of Schwann cells cultured from human peripheral nerve after transplantation into a gap within the rat sciatic nerve. J. Neurosci. 14: 1309-1319, 1994. 200-3-G6-4 (20.4)细胞PubMed: 8120626
