RA3-3A1/6.1细胞
生长状态： 悬浮生长
细胞形态： **样
**类型： IgM
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 大鼠
ATCC Number： TIB-146™
数量： 大量
RA3-3A1/6.1细胞Designations: RA3-3A1/6.1
Depositors: RL Coffman
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: RA3-3A1/6.1细胞Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the B220 mouse B cell antigen (200000 dalton form of the T200 glycoprotein, CD45R)
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Comments: Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused with the S194/5.XX0.BU.1 myeloma.
Tested and found negative for ectromelia virus (mousepox).
Propagation: RA3-3A1/6.1细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml, and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml.
References: 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
927: Coffman RL, Weissman IL. RA3-3A1/6.1细胞B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681-683, 1981. PubMed: 6970340
32948: Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902
