UCD/PR 10.11细胞
生长状态： 悬浮生长
ATCC Number： HB-8694™
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
数量： 大量
细胞形态： **样
**类型： IgG
Designations: UCD/PR 10.11
UCD/PR 10.11细胞Depositors: Regents of the University of California
Isotype: IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:UCD/PR 10.11细胞 immunoglobulin; monoclonal antibody; against type 8 and type 18 cytokeratins
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Comments: Animals were immunized with soluble extracellular cytokeratin.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts type 8 and type 18 cytokeratins in immunoblots.
The antibody can be used to detect cytokeratin in formalin or B-5 fixed paraffin sections.
The antibody primarily reacts with protein antigens in ductal portions of glands and with primary and metastatic adenocarcinomas.
UCD/PR 10.11细胞Radioactively labeled UCD/PR 10.11 antibody can also be used to detect soluble extracellular cytokeratin in a quantitative sandwich radioimmunoassay.
Propagation: ATCC complete growth medium: Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
Recommended medium UCD/PR 10.11细胞(without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3931: Cardiff RD, et al. Cytokeratin tumor markers and assays for their detection. US Patent 4,775,620 dated Oct 4 1988
