2.43细胞
是否是肿瘤细胞： 0
物种来源： 大鼠
ATCC Number： TIB-210™
数量： 大量
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG2b
运输方式： 冻存运输
Designations: 2.43
Depositors: FW Fitch
2.43细胞Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Lyt-2.2 (mouse cytotoxic, suppressor T cell antigen)
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Comments: 2.43细胞Animals were immunized with CTL Clone L3 cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 10 exp4 cells/ml and maintain between 4 x 10 exp4 and 5 x 10 exp5 cells/ml.
Preservation: 2.43细胞culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 984: Sarmiento M, et al. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing lyt 2 antigen block T-cell mediated cytolysis in the absence of complement. J. Immunol. 125: 2665-2672, 1980. PubMed: 6159417
33098: Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838
58011: Sarmiento M, et al. Cloned T lymphocytes and monoclonal antibodies as probes for cell surface molecules active in T cell-mediated cytolysis. Immunol. Rev. 68: 135-169, 1982. 2.43细胞PubMed: 6184304
