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UC8-1B9细胞

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产品名称: UC8-1B9细胞
产品型号: UC8-1B9
产品展商: HZbscience
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简单介绍

UC8-1B9细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。UC8-1B9细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


UC8-1B9细胞  的详细介绍

UC8-1B9细胞

生长状态: 悬浮生长

数量: 大量

是否是肿瘤细胞: 0

物种来源: 仓鼠

细胞形态: **样

**类型: IgG

运输方式: 冻存运输

ATCC Number: CRL-1968™

Designations: UC8-1B9

UC8-1B9细胞Depositors: JA Bluestone

Isotype: IgG

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: UC8-1B9细胞Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against dinitrophenyl (DNP)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

UC8-1B9细胞Comments: Animals were immunized with DNP derivatized KLH (keyhole limpet hemocyanin).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. UC8-1B9细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

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