219细胞
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
ATCC Number： HB-9371™
细胞形态： **样
**类型： IgG2b
Designations: 219 [Anti PDGF Hybridoma #219]
219细胞Depositors: Amgen, Inc.
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; 219细胞monoclonal antibody; against platelet derived growth factor B chain (PDGF B, v-sis form)
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Tumorigenic: Yes
Comments: Animals were immunized with affinity purified PDGF B v-sis.
Spleen cells were fused with Sp2/0-Ag14 myeloma cell.
The antibody reacts with both the A and B chains of PDGF v-sis.
It cross-reacts with the platelet form of PDGF.
Propagation: 219细胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: fetal bovine serum, 90%; DMSO, 10%
References: 41495: Thomason AR, Nicolson MA. 219细胞Methods for purification of platelet-derived growth factor. US Patent 5,175,255 dated Dec 29 1992
