UC3-10A6细胞

UC3-10A6细胞

数量： 大量

生长状态： 悬浮生长

是否是肿瘤细胞： 0

物种来源： 仓鼠

细胞形态： **样

**类型： IgG

运输方式： 冻存运输

ATCC Number： CRL-1988™

Designations: UC3-10A6

UC3-10A6细胞Depositors: JA Bluestone

Isotype: IgG

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR)

Permits/Forms: UC3-10A6细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: The antibody reacts with the murine T cell receptor gamma/delta TCR.

The antibody is specific for receptors containing the V gamma 2 segment.

Comments: Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

UC3-10A6细胞The antibody reacts with the murine T cell receptor gamma/delta TCR.

The antibody is specific for receptors containing the V gamma 2 segment.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22933: Dent AL, et al. UC3-10A6细胞Self-reactive gamma delta T cells are eliminated in the thymus. Nature 343: 714-719, 1990. PubMed: 1968227