225细胞
细胞形态： **样
**类型： IgG1 , IgG1
生长状态： 悬浮生长
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： HB-8508™
运输方式： 冻存运输
Designations: 225
Depositors: Regents of the University of California
Isotype: IgG1 , IgG1
225细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor
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Tumorigenic: Yes
Comments: Animals were immunized with partially purified EGF receptors from A-431 cells.
Spleen cells were fused with NS-1-503 myeloma cells.
225细胞The antibody reacts with EGF receptors, blocks the binding of EGF to the receptor and inhibits the growth of A-431 and T222 tumors in nude mice (although not as strongly as 528 (see ATCC HB-8509).
The antibody binds to a single class of receptor sites on A-431, HeLa and human fibroblasts, and it blocks EGF stimulated phosphorylation of membrane proteins.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; horse serum, 15%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. 225细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2040
References: 21948: Mendelsohn J, et al. Hybrid cell lines that produce monoclonal antibodies to epidermal growth factor receptor. US Patent 4,943,533 dated Jul 24 1990
92782: Sato JD, et al. 225细胞Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1: 511-529, 1983. PubMed: 6094961
