R2-9A5 [R2-9A5.2A2.1A10]细胞
生长状态： 悬浮生长
运输方式： 冻存运输
数量： 大量
**类型： IgG2b
是否是肿瘤细胞： 0
物种来源： 褐鼠
ATCC Number： CRL-2357™
Designations: R2-9A5 [R2-9A5.2A2.1A10]
R2-9A5 [R2-9A5.2A2.1A10]细胞Depositors: DH Presky
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat
Morphology:
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: R2-9A5 [R2-9A5.2A2.1A10]细胞immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis.
R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an ***** female Lewis rat inoculated with recombinant mouse IL-12 p75.
Propagation: R2-9A5 [R2-9A5.2A2.1A10]细胞ATCC complete growth medium: Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 34475: Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. R2-9A5 [R2-9A5.2A2.1A10]细胞PubMed: 8576576
