S1A.TB.4.8.2细胞
品系： BALB/c
生长状态： 悬浮生长
数量： 大量
ATCC Number： TIB-27™
相关**： **瘤
细胞类型： T**细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
细胞形态： **样
S1A.TB.4.8.2细胞Designations: S1A.TB.4.8.2 (replaces S1A.T4.G8)
Depositors: K Horibata, AW Harris
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
Permits/Forms: S1A.TB.4.8.2细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: This line was derived from the S1A lymphoma.
The cells are resistant to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Tested and found negative for ectromelia virus (mousepox).
Antigen Expression: Thy-1.2
Comments: This line was derived from the S1A lymphoma.
The cells are resistant to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: S1A.TB.4.8.2细胞Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 2 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002
Recommended serum: ATCC 30-2020
References: 22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22529: Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. S1A.TB.4.8.2细胞PubMed: 4544550
