S-S.3细胞

S-S.3细胞

细胞形态： **样

**类型： IgM; kappa light chain

数量： 大量

ATCC Number： TIB-112™

运输方式： 冻存运输

生长状态： 悬浮生长

是否是肿瘤细胞： 0

物种来源： 小鼠

Designations: S-S.3

S-S.3细胞Depositors: WC Raschke

Isotype: IgM; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against sheep red blood cell (SRBC, erythrocyte)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. S-S.3细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with SRBC.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

In 1986 this line was recloned at the ATCC from the original deposit.

Tested and found negative for ectromelia virus (mousepox).

S-S.3细胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. S-S.3细胞Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.