204-4细胞

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产品名称: 204-4细胞

产品型号: 204-4

产品展商: HZbscience

产品文档: 无相关文档

简单介绍

204-4细胞应如何避免细胞污染，细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。204-4细胞何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上之更换时间，按时更换培养基即可。

204-4细胞 的详细介绍

204-4细胞

数量：大量

生长状态：悬浮生长

运输方式：冻存运输

ATCC Number： HB-185™

细胞形态： **样

**类型： IgG2a; kappa light chain

是否是肿瘤细胞： 0

物种来源：小鼠

204-4细胞Designations: 204-4

Depositors: BS Prabhakar

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 204-4细胞immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); neutralizing

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 184).

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with a highly conserved epitope on the JVB strain of CB4.

Neutralizes most human isolates of CB4 tested.

204-4细胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.