2D7F10细胞

2D7F10细胞

是否是肿瘤细胞： 0

物种来源： 小鼠

生长状态： 悬浮生长

数量： 大量

运输方式： 冻存运输

细胞形态： **样

**类型： IgA

2D7F10细胞ATCC Number： CRL-2025™

Designations: 2D7F10

Depositors: KW Ruby

Isotype: IgA

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: 2D7F10细胞lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Leptospira pomona type kennewicki

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Tumorigenic: Yes

Comments: Animals were immunized with.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

2D7F10细胞The antibody binds to agglutinates L. interogans serovar pomona type kennewicki, and binds to type kennewicki antigen in ELISA.

It can be used to identify type kennewicki in veterinary biologicals.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 24407: . . 2D7F10细胞Proceedings of the 94th Annual Meeting of the United States Animal Health Association, Denver, CO, : 290-294, 1990.