2C4细胞
细胞形态： **样
**类型： IgG2a
ATCC Number： CRL-1760™
生长状态： 悬浮生长
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: 2C4
2C4细胞Depositors: KL Knight
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 2C4细胞immunoglobulin; monoclonal antibody; against rabbit Ia (cytotoxic)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with rabbit spleen cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
2C4细胞The antibody reacts with an isotypic determinant present on all rabbit Ia molecules, and precipitates polypeptides with molecular weights of 28000, 31000 and 35000 daltons (corresponding to the beta, Ii and alpha chains, respectively).
The antibody is cytotoxic, inhibits mixed lymphocyte reactivity by 80% and inhibits responses to LPS (but not to Con A or PHA).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; horse serum, 10%; fetal bovine serum, 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1314: Lobel SA, Knight KL. The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody. Immunology 51: 35-43, 1984. 2C4细胞PubMed: 6197360
