2C1H7 [(PTA-2846)]细胞
ATCC Number： CRL-2746™
品系： BALB/c
运输方式： 冻存运输
生长状态： 悬浮生长
器官来源： 脾
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
2C1H7 [(PTA-2846)]细胞**类型： IgG1 kappa , IgG1; kappa light chain
Designations: 2C1H7 [(PTA-2846)]
Depositors: Tularik, Inc.
Isotype: IgG1 kappa , IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Strain: BALB/c
Cell Type: 2C1H7 [(PTA-2846)]细胞hybridoma: B lymphocyte;
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Comments: Animals were immunized with a peptide derived from human beta tubulin with the sequence [H]-KATMSGVTTCLRFPGQLNA-[OH]. [57814]
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. [57814]
The antibody recognizes modified beta tubulin (an isotype of beta tubulin). It is modified at a cysteine residue at amino acid position 239. [57814]
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1 mM non-essential amino acids, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Protocol: 2C1H7 [(PTA-2846)]细胞Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/ml. Maintain cultures at a cell concentration between 2 x 105 and 5 x 105 cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: parental cell line:ATCC PTA-2686
References: 57814: 2C1H7 [(PTA-2846)]细胞Beckmann H, Edit S. Detection method for monitoring .beta. tubulin isotype specific modification. US Patent 6,306,615 dated Oct 23 2001
